Optimization of Single Cell Protein Production by Aspergillus fumigatus using a Fraction of the Full Factorial Method


Author (s):

  1. F. Ardestani, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran
  2. H.R. Ghorbani, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran
  3. M. Hezarjaribi, Shahrood Branch, Islamic Azad University, Shahrood, f.ardestani@qaemshahriau.ac.ir

Abstract:

Single cell protein has been considered as a suitable protein source to enrich the human food and animal feed. Aspergillus fumigatus PTCC5009 growth in a batch submerged culture was studied in order to determine an optimal medium composition to achieve the highest biomass production. A fraction of the full factorial method was applied for experiment design and results analysis based on signal to noise ratio statistical index. Maximum cell dry weight of 18.22 g L-1 was obtained at optimal conditions using 0.6, 0.05, 0.5 and 50 g L-1 of ammonium sulfate, iron sulfate, glycine and glucose concentrations, respectively. Glucose (32.37% contribution) and ammonium sulfate (29.25% contribution) were determined as the most effective factors on fungal biomass production. However, glycine and iron sulfate concentration had significant roles in order equal to 22.78% and 15.6%. The most interaction was diagnosed between ammonium sulfate and glycine concentrations with interaction severity index of 50.03%. An acceptable consistency of 98.75% was observed between optimum theoretical cell dry weight determined by software (18.46 g L-1) and experimentally measured one at optimal condition. A high acceptable protein content of 40.7% in dry cell biomass at the optimal condition was obtained make Aspergillus fumigates a commercial potential strain.

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