Regarding to recent scenario, wireless communication has become very pervasive. The number of mobile phones and wireless Internet users has increased significantly in recent years. Wireless networking has evolved to extend to existing wired networks. In wireless Network multiple wireless terminals jointly create and maintain Ad-hoc networks without the help of central entities. Whenever two nodes are in their radio coverage, they use single hop connection for information exchange. Whenever two nodes are not within each other’s proximity, they use multi hop connections for data exchanges. To establish multi hope connections, different types of Ad-hoc routing protocols are utilized. This paper investigates the performance of AODV, DSDV and DSR Ad-hoc routing protocols in terms of Throughput, Packet Delivery Ratio, and Average channel Throughput of the network. To achieve the satisfactory result we have used well known Network Simulator NS2 as a core simulation software.